Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways

Posted on 01/06/2022 in Research

Tabea Kohlfaerber, Mario Pieper, Michael Münter, Cornelia Holzhausen, Martin Ahrens, Christian Idel, Karl-Ludwig Bruchhage, Anke Leichtle, Peter König, Gereon Hüttmann, Hinnerk Schulz-Hildebrandt: Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways. In: Biomed. Opt. Express, vol. 13, no. 6, pp. 3211–3223, 2022.

Abstract

In the imaging of airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using fast sequences of microscopic resolution OCT (mOCT) images, OCT can use small signal fluctuations to overcome lack in contrast and speckle noise. In this way, OCT visualizes airway morphology on a cellular level and allows the tracking of the dynamic behavior of immune cells, as well as mucus transport and secretion. Here, we demonstrate that mOCT, by using temporal tissue fluctuation as contrast (dynamic mOCT), provides the possibility to study physiological and pathological tissue processes in vivo.

BibTeX (Download)

@article{Kohlfaerber:22,
title = {Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways},
author = {Tabea Kohlfaerber and Mario Pieper and Michael M\"{u}nter and Cornelia Holzhausen and Martin Ahrens and Christian Idel and Karl-Ludwig Bruchhage and Anke Leichtle and Peter K\"{o}nig and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},
url = {http://opg.optica.org/boe/abstract.cfm?URI=boe-13-6-3211},
doi = {10.1364/BOE.456104},
year  = {2022},
date = {2022-06-01},
urldate = {2022-06-01},
journal = {Biomed. Opt. Express},
volume = {13},
number = {6},
pages = {3211--3223},
publisher = {OSA},
abstract = {In the imaging of airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using fast sequences of microscopic resolution OCT (mOCT) images, OCT can use small signal fluctuations to overcome lack in contrast and speckle noise. In this way, OCT visualizes airway morphology on a cellular level and allows the tracking of the dynamic behavior of immune cells, as well as mucus transport and secretion. Here, we demonstrate that mOCT, by using temporal tissue fluctuation as contrast (dynamic mOCT), provides the possibility to study physiological and pathological tissue processes in\ vivo.},
keywords = {Full field optical coherence tomography; High nume},
pubstate = {published},
tppubtype = {article}
}