Hinnerk Schulz-Hildebrandt

@article{schulz17dynamicb,

title = {Dynamic micro-optical coherence tomography enables structural and metabolic imaging of the mammalian cochlea},

author = {Hinnerk Schulz-Hildebrandt and Svetolik Spasic and Fang Hou and Kuan-Chung Ting and Shelley Batts and Guillermo Tearney and Konstantina M Stankovic},

doi = {doi: 10.3389/fnmol.2024.1436837},

year  = {2024},

date = {2024-10-09},

journal = {Frontiers in Molecular Neuroscience},

volume = {17},

pages = {1436837},

publisher = {Frontiers},

abstract = {Sensorineural hearing loss (SNHL) is caused by damage to the mechanosensory

hair cells and auditory neurons of the cochlea. The development of imaging tools

that can directly visualize or provide functional information about a patient’s

cochlear cells is critical to identify the pathobiological defect and determine the

cells’ receptiveness to emerging SNHL treatments. However, the cochlea’s small

size, embedded location within dense bone, and sensitivity to perturbation have

historically precluded high-resolution clinical imaging. Previously, we developed

micro-optical coherence tomography (μOCT) as a platform for otologic imaging

in animal models and human cochleae. Here we report on advancing μOCT

technology to obtain simultaneously acquired and co-localized images of cell

viability/metabolic activity through dynamic μOCT (DμOCT) imaging of intracellular

motion. DμOCT obtains cross-sectional images of ATP-dependent movement of

intracellular organelles and cytoskeletal polymerization by acquiring sequential

μOCT images and computing intensity fluctuation frequency metrics on a pixelwise

basis. Using a customized benchtop DμOCT system, we demonstrate the

detailed resolution of anatomical and metabolic features of cells within the organ

of Corti, via an apical cochleostomy, in freshly-excised adult mouse cochleae.

Further, we show that DμOCT is capable of capturing rapid changes in cochlear cell

metabolism following an ototoxic insult to induce cell death and actin stabilization.

Notably, as few as 6 frames can be used to reconstruct cochlear DμOCT images

with sufficient detail to discern individual cells and their metabolic state. Taken

together, these results motivate future development of a DμOCT imaging probe

for cellular and metabolic diagnosis of SNHL in humans.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{singh2024virtual,

title = {Virtual Hall sensor triggered multi-MHz endoscopic OCT imaging for stable real-time visualization},

author = {Awanish Pratap Singh and Madita G\"{o}b and Martin Ahrens and Tim Eixmann and Berenice Schulte and Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Mark Ellrichmann and Robert Huber and Maik Rahlves},

year  = {2024},

date = {2024-01-01},

journal = {Optics Express},

volume = {32},

number = {4},

pages = {5809\textendash5825},

publisher = {Optica Publishing Group},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{harris2024reduced,

title = {Reduced sialylation of airway mucin impairs mucus transport by altering the biophysical properties of mucin},

author = {Elex S Harris and Hannah J McIntire and Marina Mazur and Hinnerk Schulz-Hildebrandt and Hui Min Leung and Guillermo J Tearney and Stefanie Krick and Steven M Rowe and Jarrod W Barnes},

year  = {2024},

date = {2024-01-01},

journal = {Scientific Reports},

volume = {14},

number = {1},

pages = {16568},

publisher = {Nature Publishing Group UK London},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dolling2024algorithm,

title = {Algorithm and software for field distortion correction in a commercial SD-OCT for corneal curvature assessment},

author = {Maron Dolling and Lara Buhl and Reginald Birngruber and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},

year  = {2024},

date = {2024-01-01},

journal = {Applied Optics},

volume = {63},

number = {10},

pages = {2694\textendash2703},

publisher = {Optica Publishing Group},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kohlfaerber:22,

title = {Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways},

author = {Tabea Kohlfaerber and Mario Pieper and Michael M\"{u}nter and Cornelia Holzhausen and Martin Ahrens and Christian Idel and Karl-Ludwig Bruchhage and Anke Leichtle and Peter K\"{o}nig and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},

url = {http://opg.optica.org/boe/abstract.cfm?URI=boe-13-6-3211},

doi = {10.1364/BOE.456104},

year  = {2022},

date = {2022-06-01},

urldate = {2022-06-01},

journal = {Biomed. Opt. Express},

volume = {13},

number = {6},

pages = {3211--3223},

publisher = {OSA},

abstract = {In the imaging of airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using fast sequences of microscopic resolution OCT (mOCT) images, OCT can use small signal fluctuations to overcome lack in contrast and speckle noise. In this way, OCT visualizes airway morphology on a cellular level and allows the tracking of the dynamic behavior of immune cells, as well as mucus transport and secretion. Here, we demonstrate that mOCT, by using temporal tissue fluctuation as contrast (dynamic mOCT), provides the possibility to study physiological and pathological tissue processes in\ vivo.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{10.1167/tvst.11.5.28,

title = {Dynamic Contrast Microscopic Optical Coherence Tomography As a Novel Method for Assessing Corneal Epithelium During Exposure to Benzalkonium Chloride},

author = {Gwen Musial and Tabea Kohlfaerber and Martin Ahrens and Hinnerk Schulz-Hildebrandt and Philipp Steven and Gereon H\"{u}ttmann},

url = {https://doi.org/10.1167/tvst.11.5.28},

doi = {10.1167/tvst.11.5.28},

issn = {2164-2591},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Translational Vision Science \& Technology},

volume = {11},

number = {5},

pages = {28-28},

abstract = { Microscopic optical coherence tomography (mOCT) has an imaging resolution of 1 µm in all voxel dimensions, but individual epithelial cells are difficult to resolve due to lack of scattering contrast. Adding dynamic contrast processing to mOCT (dmOCT) results in color images that enable visualization of individual cells and possibly give information on cellular function via the calculation of a motility coefficient. We propose this technique as a novel method of evaluating the ocular surface after exposure to a toxic chemical, benzalkonium chloride (BAK). Ex vivo cross-section images were acquired with a custom-built, frequency-domain mOCT system. Eyes were explanted from healthy adult C57BL/6 mice and imaged every 30 minutes with five sets of dmOCT scans at each imaging time. Total epithelium and stroma thicknesses were measured from a single mOCT B-scan, and measures of color changes (hue) and the motility coefficient were acquired from dmOCT scans. After 30-minute exposures to 0.005% BAK, local motility decreased and total epithelium thickness increased compared to controls. For basal epithelium cells, local motility decreased after 60-minute exposures, and the hue shifted red after 90-minute exposures. Stroma thickness did not significantly swell until 150-minute exposures to BAK. dmOCT allows us to view the behavior of the cornea epithelium under toxic stress due to BAK, revealing parallel swelling of the extracellular matrix and changes in local subcellular motion. The evaluation of the cornea epithelium using dmOCT is helpful to our understanding of the toxic effects of BAK. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}