2025
|
Hinnerk Schulz-Hildebrandt; Michael Wang-Evers; Naja Meyer-Schell; Daniel Karasik; Malte J Casper; Tim Eixmann; Felix Hilge; Reginald Birngruber; Dieter Manstein; Gereon Hüttmann Optical coherence tomography needle probe for real-time visualization of temperature-induced phase changes within subcutaneous fatty tissue Journal Article In: Journal of Biomedical Optics, vol. 30, no. 3, pp. 035002–035002, 2025. @article{schulz2025optical,
title = {Optical coherence tomography needle probe for real-time visualization of temperature-induced phase changes within subcutaneous fatty tissue},
author = {Hinnerk Schulz-Hildebrandt and Michael Wang-Evers and Naja Meyer-Schell and Daniel Karasik and Malte J Casper and Tim Eixmann and Felix Hilge and Reginald Birngruber and Dieter Manstein and Gereon H\"{u}ttmann},
year = {2025},
date = {2025-01-01},
urldate = {2025-01-01},
journal = {Journal of Biomedical Optics},
volume = {30},
number = {3},
pages = {035002\textendash035002},
publisher = {Society of Photo-Optical Instrumentation Engineers},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2024
|
Hinnerk Schulz-Hildebrandt; Svetolik Spasic; Fang Hou; Kuan-Chung Ting; Shelley Batts; Guillermo Tearney; Konstantina M Stankovic Dynamic micro-optical coherence tomography enables structural and metabolic imaging of the mammalian cochlea Journal Article In: Frontiers in Molecular Neuroscience, vol. 17, pp. 1436837, 2024. @article{schulz17dynamicb,
title = {Dynamic micro-optical coherence tomography enables structural and metabolic imaging of the mammalian cochlea},
author = {Hinnerk Schulz-Hildebrandt and Svetolik Spasic and Fang Hou and Kuan-Chung Ting and Shelley Batts and Guillermo Tearney and Konstantina M Stankovic},
doi = {doi: 10.3389/fnmol.2024.1436837},
year = {2024},
date = {2024-10-09},
journal = {Frontiers in Molecular Neuroscience},
volume = {17},
pages = {1436837},
publisher = {Frontiers},
abstract = {Sensorineural hearing loss (SNHL) is caused by damage to the mechanosensory
hair cells and auditory neurons of the cochlea. The development of imaging tools
that can directly visualize or provide functional information about a patient’s
cochlear cells is critical to identify the pathobiological defect and determine the
cells’ receptiveness to emerging SNHL treatments. However, the cochlea’s small
size, embedded location within dense bone, and sensitivity to perturbation have
historically precluded high-resolution clinical imaging. Previously, we developed
micro-optical coherence tomography (μOCT) as a platform for otologic imaging
in animal models and human cochleae. Here we report on advancing μOCT
technology to obtain simultaneously acquired and co-localized images of cell
viability/metabolic activity through dynamic μOCT (DμOCT) imaging of intracellular
motion. DμOCT obtains cross-sectional images of ATP-dependent movement of
intracellular organelles and cytoskeletal polymerization by acquiring sequential
μOCT images and computing intensity fluctuation frequency metrics on a pixelwise
basis. Using a customized benchtop DμOCT system, we demonstrate the
detailed resolution of anatomical and metabolic features of cells within the organ
of Corti, via an apical cochleostomy, in freshly-excised adult mouse cochleae.
Further, we show that DμOCT is capable of capturing rapid changes in cochlear cell
metabolism following an ototoxic insult to induce cell death and actin stabilization.
Notably, as few as 6 frames can be used to reconstruct cochlear DμOCT images
with sufficient detail to discern individual cells and their metabolic state. Taken
together, these results motivate future development of a DμOCT imaging probe
for cellular and metabolic diagnosis of SNHL in humans.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Sensorineural hearing loss (SNHL) is caused by damage to the mechanosensory
hair cells and auditory neurons of the cochlea. The development of imaging tools
that can directly visualize or provide functional information about a patient’s
cochlear cells is critical to identify the pathobiological defect and determine the
cells’ receptiveness to emerging SNHL treatments. However, the cochlea’s small
size, embedded location within dense bone, and sensitivity to perturbation have
historically precluded high-resolution clinical imaging. Previously, we developed
micro-optical coherence tomography (μOCT) as a platform for otologic imaging
in animal models and human cochleae. Here we report on advancing μOCT
technology to obtain simultaneously acquired and co-localized images of cell
viability/metabolic activity through dynamic μOCT (DμOCT) imaging of intracellular
motion. DμOCT obtains cross-sectional images of ATP-dependent movement of
intracellular organelles and cytoskeletal polymerization by acquiring sequential
μOCT images and computing intensity fluctuation frequency metrics on a pixelwise
basis. Using a customized benchtop DμOCT system, we demonstrate the
detailed resolution of anatomical and metabolic features of cells within the organ
of Corti, via an apical cochleostomy, in freshly-excised adult mouse cochleae.
Further, we show that DμOCT is capable of capturing rapid changes in cochlear cell
metabolism following an ototoxic insult to induce cell death and actin stabilization.
Notably, as few as 6 frames can be used to reconstruct cochlear DμOCT images
with sufficient detail to discern individual cells and their metabolic state. Taken
together, these results motivate future development of a DμOCT imaging probe
for cellular and metabolic diagnosis of SNHL in humans. |
Awanish Pratap Singh; Madita Göb; Martin Ahrens; Tim Eixmann; Berenice Schulte; Hinnerk Schulz-Hildebrandt; Gereon Hüttmann; Mark Ellrichmann; Robert Huber; Maik Rahlves Virtual Hall sensor triggered multi-MHz endoscopic OCT imaging for stable real-time visualization Journal Article In: Optics Express, vol. 32, no. 4, pp. 5809–5825, 2024. @article{singh2024virtual,
title = {Virtual Hall sensor triggered multi-MHz endoscopic OCT imaging for stable real-time visualization},
author = {Awanish Pratap Singh and Madita G\"{o}b and Martin Ahrens and Tim Eixmann and Berenice Schulte and Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Mark Ellrichmann and Robert Huber and Maik Rahlves},
year = {2024},
date = {2024-01-01},
journal = {Optics Express},
volume = {32},
number = {4},
pages = {5809\textendash5825},
publisher = {Optica Publishing Group},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Elex S Harris; Hannah J McIntire; Marina Mazur; Hinnerk Schulz-Hildebrandt; Hui Min Leung; Guillermo J Tearney; Stefanie Krick; Steven M Rowe; Jarrod W Barnes Reduced sialylation of airway mucin impairs mucus transport by altering the biophysical properties of mucin Journal Article In: Scientific Reports, vol. 14, no. 1, pp. 16568, 2024. @article{harris2024reduced,
title = {Reduced sialylation of airway mucin impairs mucus transport by altering the biophysical properties of mucin},
author = {Elex S Harris and Hannah J McIntire and Marina Mazur and Hinnerk Schulz-Hildebrandt and Hui Min Leung and Guillermo J Tearney and Stefanie Krick and Steven M Rowe and Jarrod W Barnes},
year = {2024},
date = {2024-01-01},
journal = {Scientific Reports},
volume = {14},
number = {1},
pages = {16568},
publisher = {Nature Publishing Group UK London},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Maron Dolling; Lara Buhl; Reginald Birngruber; Gereon Hüttmann; Hinnerk Schulz-Hildebrandt Algorithm and software for field distortion correction in a commercial SD-OCT for corneal curvature assessment Journal Article In: Applied Optics, vol. 63, no. 10, pp. 2694–2703, 2024. @article{dolling2024algorithm,
title = {Algorithm and software for field distortion correction in a commercial SD-OCT for corneal curvature assessment},
author = {Maron Dolling and Lara Buhl and Reginald Birngruber and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},
year = {2024},
date = {2024-01-01},
journal = {Applied Optics},
volume = {63},
number = {10},
pages = {2694\textendash2703},
publisher = {Optica Publishing Group},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2023
|
M Sieren; S Jaeckle; T Eixmann; H Schulz-Hildebrandt; M Preuss; V García-Vázquez; E Stahlberg; M Kleemann; J Barkhausen; J Goltz; others Strahlenfreie Implantation eines thorakalen Aortenstentgrafts mittels faseroptischen und elektromagnetischen Trackings: Eine Phantomstudie Proceedings Article In: RöFo-Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren, pp. ab83, Georg Thieme Verlag 2023. @inproceedings{sieren2022strahlenfreie,
title = {Strahlenfreie Implantation eines thorakalen Aortenstentgrafts mittels faseroptischen und elektromagnetischen Trackings: Eine Phantomstudie},
author = {M Sieren and S Jaeckle and T Eixmann and H Schulz-Hildebrandt and M Preuss and V Garc\'{i}a-V\'{a}zquez and E Stahlberg and M Kleemann and J Barkhausen and J Goltz and others},
year = {2023},
date = {2023-01-01},
urldate = {2022-01-01},
booktitle = {R\"{o}Fo-Fortschritte auf dem Gebiet der R\"{o}ntgenstrahlen und der bildgebenden Verfahren},
volume = {194},
number = {S 01},
pages = {ab83},
organization = {Georg Thieme Verlag},
keywords = {},
pubstate = {published},
tppubtype = {inproceedings}
}
|
Anke Leichtle; Zuzana Penxova; Thorge Kempin; David Leffers; Martin Ahrens; Peter König; Ralf Brinkmann; Gereon Hüttmann; Karl-Ludwig Bruchhage; Hinnerk Schulz-Hildebrandt Dynamic microscopic optical coherence tomography as a new diagnostic tool for otitis media Proceedings Article In: Photonics, pp. 685, MDPI 2023. @inproceedings{leichtle2023dynamic,
title = {Dynamic microscopic optical coherence tomography as a new diagnostic tool for otitis media},
author = {Anke Leichtle and Zuzana Penxova and Thorge Kempin and David Leffers and Martin Ahrens and Peter K\"{o}nig and Ralf Brinkmann and Gereon H\"{u}ttmann and Karl-Ludwig Bruchhage and Hinnerk Schulz-Hildebrandt},
year = {2023},
date = {2023-01-01},
booktitle = {Photonics},
volume = {10},
number = {6},
pages = {685},
organization = {MDPI},
keywords = {},
pubstate = {published},
tppubtype = {inproceedings}
}
|
2022
|
Tabea Kohlfaerber; Mario Pieper; Michael Münter; Cornelia Holzhausen; Martin Ahrens; Christian Idel; Karl-Ludwig Bruchhage; Anke Leichtle; Peter König; Gereon Hüttmann; Hinnerk Schulz-Hildebrandt Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways Journal Article In: Biomed. Opt. Express, vol. 13, no. 6, pp. 3211–3223, 2022. @article{Kohlfaerber:22,
title = {Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways},
author = {Tabea Kohlfaerber and Mario Pieper and Michael M\"{u}nter and Cornelia Holzhausen and Martin Ahrens and Christian Idel and Karl-Ludwig Bruchhage and Anke Leichtle and Peter K\"{o}nig and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},
url = {http://opg.optica.org/boe/abstract.cfm?URI=boe-13-6-3211},
doi = {10.1364/BOE.456104},
year = {2022},
date = {2022-06-01},
urldate = {2022-06-01},
journal = {Biomed. Opt. Express},
volume = {13},
number = {6},
pages = {3211--3223},
publisher = {OSA},
abstract = {In the imaging of airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using fast sequences of microscopic resolution OCT (mOCT) images, OCT can use small signal fluctuations to overcome lack in contrast and speckle noise. In this way, OCT visualizes airway morphology on a cellular level and allows the tracking of the dynamic behavior of immune cells, as well as mucus transport and secretion. Here, we demonstrate that mOCT, by using temporal tissue fluctuation as contrast (dynamic mOCT), provides the possibility to study physiological and pathological tissue processes in\ vivo.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the imaging of airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using fast sequences of microscopic resolution OCT (mOCT) images, OCT can use small signal fluctuations to overcome lack in contrast and speckle noise. In this way, OCT visualizes airway morphology on a cellular level and allows the tracking of the dynamic behavior of immune cells, as well as mucus transport and secretion. Here, we demonstrate that mOCT, by using temporal tissue fluctuation as contrast (dynamic mOCT), provides the possibility to study physiological and pathological tissue processes in vivo. |
Tabea Kohlfaerber; Mario Pieper; Michael Münter; Cornelia Holzhausen; Martin Ahrens; Christian Idel; Karl Ludwig Bruchhage; Anke Leichtle; Peter König; Gereon Hüttmann; Hinnerk Schulz-Hildebrandt Dynamic microscopic optical coherence tomography to visualize morphological and functional micro-anatomy of the airways Miscellaneous 2022. @misc{kohlfaerber2022dynamic,
title = {Dynamic microscopic optical coherence tomography to visualize morphological and functional micro-anatomy of the airways},
author = {Tabea Kohlfaerber and Mario Pieper and Michael M\"{u}nter and Cornelia Holzhausen and Martin Ahrens and Christian Idel and Karl Ludwig Bruchhage and Anke Leichtle and Peter K\"{o}nig and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},
year = {2022},
date = {2022-01-01},
urldate = {2022-01-01},
keywords = {},
pubstate = {published},
tppubtype = {misc}
}
|
Sonja Jäckle; Tim Eixmann; Florian Matysiak; Malte M Sieren; Marco Horn; Hinnerk Schulz-Hildebrandt; Gereon Hüttmann; Torben Pätz Abstract: 3D Stent Graft Guidance Based on Tracking Systems Proceedings Article In: Maier-Hein, Klaus; Deserno, Thomas M; Handels, Heinz; Maier, Andreas; Palm, Christoph; Tolxdorff, Thomas (Ed.): Bildverarbeitung für die Medizin 2022, pp. 253, Springer Fachmedien Wiesbaden, Wiesbaden, 2022, ISBN: 978-3-658-36932-3. @inproceedings{10.1007/978-3-658-36932-3_53,
title = {Abstract: 3D Stent Graft Guidance Based on Tracking Systems},
author = {Sonja J\"{a}ckle and Tim Eixmann and Florian Matysiak and Malte M Sieren and Marco Horn and Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Torben P\"{a}tz},
editor = {Klaus Maier-Hein and Thomas M Deserno and Heinz Handels and Andreas Maier and Christoph Palm and Thomas Tolxdorff},
isbn = {978-3-658-36932-3},
year = {2022},
date = {2022-01-01},
booktitle = {Bildverarbeitung f\"{u}r die Medizin 2022},
pages = {253},
publisher = {Springer Fachmedien Wiesbaden},
address = {Wiesbaden},
abstract = {In endovascular aneurysm repair (EVAR) procedures, the stent graft navigation and implantation is currently performed under a two-dimensional (2D) imaging-based guidance requiring X-rays and contrast agent. In [1], a novel three-dimensional (3D) stent graft guidance approach based on tracking systems is introduced. The method is based on a 3D guidance method which combines fiber optical shape sensing with electromagnetic tracking to obtain the 3D shape [2] of the tracked instrument, e.g., a stent graft system. In this work, the approach is extended to provide also the 3D stent graft shape.},
keywords = {},
pubstate = {published},
tppubtype = {inproceedings}
}
In endovascular aneurysm repair (EVAR) procedures, the stent graft navigation and implantation is currently performed under a two-dimensional (2D) imaging-based guidance requiring X-rays and contrast agent. In [1], a novel three-dimensional (3D) stent graft guidance approach based on tracking systems is introduced. The method is based on a 3D guidance method which combines fiber optical shape sensing with electromagnetic tracking to obtain the 3D shape [2] of the tracked instrument, e.g., a stent graft system. In this work, the approach is extended to provide also the 3D stent graft shape. |