2025
|
Cornelia Holzhausen; Hinnerk Schulz-Hildebrandt; Martin Ahrens; Noah Heldt; Mario Pieper; Heike Biller; Sönke Von Weihe; David Ellebrecht; Mustafa Abdo; Stefan Steurer; Christoph Fraune; Klaus F. Rabe; Gereon Hüttmann; Peter König Imaging of human airways by endoscope-compatible dynamic microscopic optical coherence tomography Journal Article In: Frontiers in Medicine, vol. 12, 2025, (Article in Section Translational Medicine). @article{Holzhausen2025,
title = {Imaging of human airways by endoscope-compatible dynamic microscopic optical coherence tomography},
author = {Cornelia Holzhausen and Hinnerk Schulz-Hildebrandt and Martin Ahrens and Noah Heldt and Mario Pieper and Heike Biller and S\"{o}nke Von Weihe and David Ellebrecht and Mustafa Abdo and Stefan Steurer and Christoph Fraune and Klaus F. Rabe and Gereon H\"{u}ttmann and Peter K\"{o}nig},
url = {https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2025.1658890},
doi = {10.3389/fmed.2025.1658890},
year = {2025},
date = {2025-09-30},
urldate = {2025-01-01},
journal = {Frontiers in Medicine},
volume = {12},
note = {Article in Section Translational Medicine},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Hinnerk Schulz-Hildebrandt When Purple Perceived Only at Fixation: A Fixation and Distance-Dependent Color Illusion Miscellaneous Forthcoming Forthcoming. @misc{schulzhildebrandt2025purpleperceivedfixationfixationb,
title = {When Purple Perceived Only at Fixation: A Fixation and Distance-Dependent Color Illusion},
author = {Hinnerk Schulz-Hildebrandt},
url = {https://arxiv.org/abs/2509.11582},
year = {2025},
date = {2025-09-16},
urldate = {2025-01-01},
keywords = {},
pubstate = {forthcoming},
tppubtype = {misc}
}
|
Burhan, Sazgar; Göb, Madita; Pieper, Mario; Laedtke, Tjalfe; Grahl, Thorge; Münter, Michael; Schulz-Hildebrandt, Hinnerk; Hüttmann, Gereon; König, Peter; Huber, Robert Label-free volumetric imaging of porcine kidney tissue over extended areas using dynamic MHz-OCT Journal Article In: Scientific Reports, vol. 15, pp. 32426, 2025. @article{nokey,
title = {Label-free volumetric imaging of porcine kidney tissue over extended areas using dynamic MHz-OCT},
author = {Burhan, Sazgar and G\"{o}b, Madita and Pieper, Mario and Laedtke, Tjalfe and Grahl, Thorge and M\"{u}nter, Michael and Schulz-Hildebrandt, Hinnerk and H\"{u}ttmann, Gereon and K\"{o}nig, Peter and Huber, Robert},
doi = {10.1038/s41598-025-15032-6},
year = {2025},
date = {2025-09-12},
journal = {Scientific Reports},
volume = {15},
pages = {32426},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Felix Hilge; Michael Wang-Evers; Lara Buhl; Heather Downs; Maron Dolling; Lukas Pohl; Reginald Birngruber; Hinnerk Schulz-Hildebrandt; Gereon Hüttmann; Dieter Manstein Label-free visualization and quantitative analysis of Far UV-C skin safety with dynamic optical coherence tomography with subcellular resolution Journal Article In: Biomed. Opt. Express, vol. 16, no. 9, pp. 3682–3701, 2025. @article{Hilge:25,
title = {Label-free visualization and quantitative analysis of Far UV-C skin safety with dynamic optical coherence tomography with subcellular resolution},
author = {Felix Hilge and Michael Wang-Evers and Lara Buhl and Heather Downs and Maron Dolling and Lukas Pohl and Reginald Birngruber and Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Dieter Manstein},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-16-9-3682},
doi = {10.1364/BOE.569995},
year = {2025},
date = {2025-09-01},
urldate = {2025-09-01},
journal = {Biomed. Opt. Express},
volume = {16},
number = {9},
pages = {3682\textendash3701},
publisher = {Optica Publishing Group},
abstract = {The recent COVID-19 pandemic sparked interest in interventional public health measures like ultraviolet germicidal irradiation (UVGI) of occupied spaces with the development of Far UV-C (200\textendash230\ nm) emitting sources promising similar antimicrobial properties as conventional (254 nm) disinfecting lamps without adverse effects on human skin. When investigating the impact of different UV irradiation parameters, the visualization of cellular damage, like apoptosis and formation of photoproducts, currently requires immunohistochemical tissue processing and is of an invasive nature. Dynamic-microscopic optical coherence tomography (dmOCT) is a non-invasive technique that generates label-free images based on the dynamic scattering properties of cells. In this study, we expose an in-vitro human skin model to either UV-A/B or Far UV-C to demonstrate the ability of dmOCT to visualize cell death, followed by UV-induced photodamage, and perform immunohistochemical analysis. Our results clearly show a change in dynamic contrast within the viable epidermis and changes in the morphology of keratinocyte nuclei after UV-A/B exposure with 250 mJ/cm2, which were observable in the histology as well. In contrast, exposure to 2000 mJ/cm2 of Far UV-C did not have an effect on keratinocytes within the epidermis in either the dmOCT imaging or the immunohistochemistry experiment. In addition to demonstrating Far UV-C skin safety, these findings highlight the potential of dmOCT to assess tissue damage and viability non-invasively.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The recent COVID-19 pandemic sparked interest in interventional public health measures like ultraviolet germicidal irradiation (UVGI) of occupied spaces with the development of Far UV-C (200–230 nm) emitting sources promising similar antimicrobial properties as conventional (254 nm) disinfecting lamps without adverse effects on human skin. When investigating the impact of different UV irradiation parameters, the visualization of cellular damage, like apoptosis and formation of photoproducts, currently requires immunohistochemical tissue processing and is of an invasive nature. Dynamic-microscopic optical coherence tomography (dmOCT) is a non-invasive technique that generates label-free images based on the dynamic scattering properties of cells. In this study, we expose an in-vitro human skin model to either UV-A/B or Far UV-C to demonstrate the ability of dmOCT to visualize cell death, followed by UV-induced photodamage, and perform immunohistochemical analysis. Our results clearly show a change in dynamic contrast within the viable epidermis and changes in the morphology of keratinocyte nuclei after UV-A/B exposure with 250 mJ/cm2, which were observable in the histology as well. In contrast, exposure to 2000 mJ/cm2 of Far UV-C did not have an effect on keratinocytes within the epidermis in either the dmOCT imaging or the immunohistochemistry experiment. In addition to demonstrating Far UV-C skin safety, these findings highlight the potential of dmOCT to assess tissue damage and viability non-invasively. |
Hinnerk Schulz-Hildebrandt; Joseph A. Gardecki; Tiffany Miller; Maria Avila-Wallace; Erica Villareyna-Lopez; Guillermo Tearney Phase-sensitive dynamic micro-optical coherence tomography for high-speed intracellular motion imaging Journal Article In: Opt. Lett., vol. 50, no. 15, pp. 4734–4737, 2025. @article{Schulz-Hildebrandt:25,
title = {Phase-sensitive dynamic micro-optical coherence tomography for high-speed intracellular motion imaging},
author = {Hinnerk Schulz-Hildebrandt and Joseph A. Gardecki and Tiffany Miller and Maria Avila-Wallace and Erica Villareyna-Lopez and Guillermo Tearney},
url = {https://opg.optica.org/ol/abstract.cfm?URI=ol-50-15-4734},
doi = {10.1364/OL.563024},
year = {2025},
date = {2025-08-01},
urldate = {2025-08-01},
journal = {Opt. Lett.},
volume = {50},
number = {15},
pages = {4734\textendash4737},
publisher = {Optica Publishing Group},
abstract = {Dynamic optical coherence tomography with micrometer resolution (DµOCT) offers enhanced contrast information by evaluating time-dependent signal fluctuations in images of living tissue. DµOCT\'s use has been limited to imaging excised fresh tissue specimens or 3D cell cultures due to the long observation times required, typically ranging from 1.35 to 25 seconds. To reduce the time needed to obtain DµOCT images, we developed a phase-based algorithm that analyzes intracellular motion by measuring phase changes between adjacent B-scans. This approach significantly reduces imaging time to as low as 40.5 ms while providing a quantitative measure of intracellular motion.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Dynamic optical coherence tomography with micrometer resolution (DµOCT) offers enhanced contrast information by evaluating time-dependent signal fluctuations in images of living tissue. DµOCT's use has been limited to imaging excised fresh tissue specimens or 3D cell cultures due to the long observation times required, typically ranging from 1.35 to 25 seconds. To reduce the time needed to obtain DµOCT images, we developed a phase-based algorithm that analyzes intracellular motion by measuring phase changes between adjacent B-scans. This approach significantly reduces imaging time to as low as 40.5 ms while providing a quantitative measure of intracellular motion. |
Hinnerk Schulz-Hildebrandt; Michael Wang-Evers; Naja Meyer-Schell; Daniel Karasik; Malte J Casper; Tim Eixmann; Felix Hilge; Reginald Birngruber; Dieter Manstein; Gereon Hüttmann Optical coherence tomography needle probe for real-time visualization of temperature-induced phase changes within subcutaneous fatty tissue Journal Article In: Journal of Biomedical Optics, vol. 30, no. 3, pp. 035002–035002, 2025. @article{schulz2025optical,
title = {Optical coherence tomography needle probe for real-time visualization of temperature-induced phase changes within subcutaneous fatty tissue},
author = {Hinnerk Schulz-Hildebrandt and Michael Wang-Evers and Naja Meyer-Schell and Daniel Karasik and Malte J Casper and Tim Eixmann and Felix Hilge and Reginald Birngruber and Dieter Manstein and Gereon H\"{u}ttmann},
year = {2025},
date = {2025-01-01},
urldate = {2025-01-01},
journal = {Journal of Biomedical Optics},
volume = {30},
number = {3},
pages = {035002\textendash035002},
publisher = {Society of Photo-Optical Instrumentation Engineers},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2024
|
Hinnerk Schulz-Hildebrandt; Svetolik Spasic; Fang Hou; Kuan-Chung Ting; Shelley Batts; Guillermo Tearney; Konstantina M Stankovic Dynamic micro-optical coherence tomography enables structural and metabolic imaging of the mammalian cochlea Journal Article In: Frontiers in Molecular Neuroscience, vol. 17, pp. 1436837, 2024. @article{schulz17dynamicb,
title = {Dynamic micro-optical coherence tomography enables structural and metabolic imaging of the mammalian cochlea},
author = {Hinnerk Schulz-Hildebrandt and Svetolik Spasic and Fang Hou and Kuan-Chung Ting and Shelley Batts and Guillermo Tearney and Konstantina M Stankovic},
doi = {doi: 10.3389/fnmol.2024.1436837},
year = {2024},
date = {2024-10-09},
journal = {Frontiers in Molecular Neuroscience},
volume = {17},
pages = {1436837},
publisher = {Frontiers},
abstract = {Sensorineural hearing loss (SNHL) is caused by damage to the mechanosensory
hair cells and auditory neurons of the cochlea. The development of imaging tools
that can directly visualize or provide functional information about a patient’s
cochlear cells is critical to identify the pathobiological defect and determine the
cells’ receptiveness to emerging SNHL treatments. However, the cochlea’s small
size, embedded location within dense bone, and sensitivity to perturbation have
historically precluded high-resolution clinical imaging. Previously, we developed
micro-optical coherence tomography (μOCT) as a platform for otologic imaging
in animal models and human cochleae. Here we report on advancing μOCT
technology to obtain simultaneously acquired and co-localized images of cell
viability/metabolic activity through dynamic μOCT (DμOCT) imaging of intracellular
motion. DμOCT obtains cross-sectional images of ATP-dependent movement of
intracellular organelles and cytoskeletal polymerization by acquiring sequential
μOCT images and computing intensity fluctuation frequency metrics on a pixelwise
basis. Using a customized benchtop DμOCT system, we demonstrate the
detailed resolution of anatomical and metabolic features of cells within the organ
of Corti, via an apical cochleostomy, in freshly-excised adult mouse cochleae.
Further, we show that DμOCT is capable of capturing rapid changes in cochlear cell
metabolism following an ototoxic insult to induce cell death and actin stabilization.
Notably, as few as 6 frames can be used to reconstruct cochlear DμOCT images
with sufficient detail to discern individual cells and their metabolic state. Taken
together, these results motivate future development of a DμOCT imaging probe
for cellular and metabolic diagnosis of SNHL in humans.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Sensorineural hearing loss (SNHL) is caused by damage to the mechanosensory
hair cells and auditory neurons of the cochlea. The development of imaging tools
that can directly visualize or provide functional information about a patient’s
cochlear cells is critical to identify the pathobiological defect and determine the
cells’ receptiveness to emerging SNHL treatments. However, the cochlea’s small
size, embedded location within dense bone, and sensitivity to perturbation have
historically precluded high-resolution clinical imaging. Previously, we developed
micro-optical coherence tomography (μOCT) as a platform for otologic imaging
in animal models and human cochleae. Here we report on advancing μOCT
technology to obtain simultaneously acquired and co-localized images of cell
viability/metabolic activity through dynamic μOCT (DμOCT) imaging of intracellular
motion. DμOCT obtains cross-sectional images of ATP-dependent movement of
intracellular organelles and cytoskeletal polymerization by acquiring sequential
μOCT images and computing intensity fluctuation frequency metrics on a pixelwise
basis. Using a customized benchtop DμOCT system, we demonstrate the
detailed resolution of anatomical and metabolic features of cells within the organ
of Corti, via an apical cochleostomy, in freshly-excised adult mouse cochleae.
Further, we show that DμOCT is capable of capturing rapid changes in cochlear cell
metabolism following an ototoxic insult to induce cell death and actin stabilization.
Notably, as few as 6 frames can be used to reconstruct cochlear DμOCT images
with sufficient detail to discern individual cells and their metabolic state. Taken
together, these results motivate future development of a DμOCT imaging probe
for cellular and metabolic diagnosis of SNHL in humans. |
Awanish Pratap Singh; Madita Göb; Martin Ahrens; Tim Eixmann; Berenice Schulte; Hinnerk Schulz-Hildebrandt; Gereon Hüttmann; Mark Ellrichmann; Robert Huber; Maik Rahlves Virtual Hall sensor triggered multi-MHz endoscopic OCT imaging for stable real-time visualization Journal Article In: Optics Express, vol. 32, no. 4, pp. 5809–5825, 2024. @article{singh2024virtual,
title = {Virtual Hall sensor triggered multi-MHz endoscopic OCT imaging for stable real-time visualization},
author = {Awanish Pratap Singh and Madita G\"{o}b and Martin Ahrens and Tim Eixmann and Berenice Schulte and Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Mark Ellrichmann and Robert Huber and Maik Rahlves},
year = {2024},
date = {2024-01-01},
journal = {Optics Express},
volume = {32},
number = {4},
pages = {5809\textendash5825},
publisher = {Optica Publishing Group},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Elex S Harris; Hannah J McIntire; Marina Mazur; Hinnerk Schulz-Hildebrandt; Hui Min Leung; Guillermo J Tearney; Stefanie Krick; Steven M Rowe; Jarrod W Barnes Reduced sialylation of airway mucin impairs mucus transport by altering the biophysical properties of mucin Journal Article In: Scientific Reports, vol. 14, no. 1, pp. 16568, 2024. @article{harris2024reduced,
title = {Reduced sialylation of airway mucin impairs mucus transport by altering the biophysical properties of mucin},
author = {Elex S Harris and Hannah J McIntire and Marina Mazur and Hinnerk Schulz-Hildebrandt and Hui Min Leung and Guillermo J Tearney and Stefanie Krick and Steven M Rowe and Jarrod W Barnes},
year = {2024},
date = {2024-01-01},
journal = {Scientific Reports},
volume = {14},
number = {1},
pages = {16568},
publisher = {Nature Publishing Group UK London},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Maron Dolling; Lara Buhl; Reginald Birngruber; Gereon Hüttmann; Hinnerk Schulz-Hildebrandt Algorithm and software for field distortion correction in a commercial SD-OCT for corneal curvature assessment Journal Article In: Applied Optics, vol. 63, no. 10, pp. 2694–2703, 2024. @article{dolling2024algorithm,
title = {Algorithm and software for field distortion correction in a commercial SD-OCT for corneal curvature assessment},
author = {Maron Dolling and Lara Buhl and Reginald Birngruber and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},
year = {2024},
date = {2024-01-01},
journal = {Applied Optics},
volume = {63},
number = {10},
pages = {2694\textendash2703},
publisher = {Optica Publishing Group},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|