Abstract
While optical coherence tomography (OCT) provides a resolution downto 1 textmum, it has difficulties in visualizing cellular structures due to alack of scattering contrast. By evaluating signal fluctuations, asignificant contrast enhancement was demonstrated using time-domainfull-field OCT (FF-OCT), which makes cellular and subcellular structuresvisible. The putative cause of the dynamic OCT signal is thesite-dependent active motion of cellular structures in a sub-micrometerrange, which provides histology-like contrast. Here we demonstrate dynamiccontrast with a scanning frequency-domain OCT (FD-OCT), which we believehas crucial advantages. Given the inherent sectional imaging geometry,scanning FD-OCT provides depth-resolved images across tissue layers, aperspective known from histopathology, much faster and more efficientlythan FF-OCT. Both shorter acquisition times and tomographicdepth-sectioning reduce the sensitivity of dynamic contrast for bulktissue motion artifacts and simplify their correction in post-processing.Dynamic contrast makes microscopic FD-OCT a promising tool for thehistological analysis of unstained tissues.
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@article{Munter:20,
title = {Dynamic contrast in scanning microscopic OCT},
author = {Michael M\"{u}nter and Malte vom Endt and Mario Pieper and Malte Casper and Martin Ahrens and Tabea Kohlfaerber and Ramtin Rahmanzadeh and Peter K\"{o}nig and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},
url = {http://ol.osa.org/abstract.cfm?URI=ol-45-17-4766},
doi = {10.1364/OL.396134},
year = {2020},
date = {2020-09-01},
journal = {Opt. Lett.},
volume = {45},
number = {17},
pages = {4766--4769},
publisher = {OSA},
abstract = {While optical coherence tomography (OCT) provides a resolution downto 1 textmum, it has difficulties in visualizing cellular structures due to alack of scattering contrast. By evaluating signal fluctuations, asignificant contrast enhancement was demonstrated using time-domainfull-field OCT (FF-OCT), which makes cellular and subcellular structuresvisible. The putative cause of the dynamic OCT signal is thesite-dependent active motion of cellular structures in a sub-micrometerrange, which provides histology-like contrast. Here we demonstrate dynamiccontrast with a scanning frequency-domain OCT (FD-OCT), which we believehas crucial advantages. Given the inherent sectional imaging geometry,scanning FD-OCT provides depth-resolved images across tissue layers, aperspective known from histopathology, much faster and more efficientlythan FF-OCT. Both shorter acquisition times and tomographicdepth-sectioning reduce the sensitivity of dynamic contrast for bulktissue motion artifacts and simplify their correction in post-processing.Dynamic contrast makes microscopic FD-OCT a promising tool for thehistological analysis of unstained tissues.},
keywords = {Full field optical coherence tomography; Image processing; Image quality; Line scan cameras; Optical coherence tomography; Tomography, mOCT, Optical coherence tomography},
pubstate = {published},
tppubtype = {article}
}